منابع مشابه
Over Expression of Influenza Virus M2 Protein in Prokaryotic System
Background and Aims: Influenza A virus of Orthomyxoviridae family is able to create pandemic influenza. Vaccination is the most effective way to prevent influenza virus infection. Matrix protein 2 (M2) is a homotetramer ion channel with 97 amino acids length and highly conserved among influenza viruses and is considered for development of a universal influenza vaccine. Materials and Methods: We...
متن کاملHeterologous expression of maize (Zea mays L.) Mir1 cysteine proteinase in eukaryotic and prokaryotic expression systems.
Several heterologous expression systems were tested for their ability to express a unique maize cysteine proteinase Mir1. A baculovirus-based expression system using Trichoplusia ni larvae as host resulted in the expression of Mir1 that was correctly processed and exhibited proteinase activity. Expression in Escherichia coli resulted in accumulation of Mir1, but it had limited solubility and en...
متن کاملProkaryotic expression and purification of porcine Sox6
Wanxue WEN*, Xiaoling CHEN*, Zhiqing HUANG**, Meng XU, Daiwen CHEN, Bing YU, Jun HE, Junqiu LUO, Xiangbing MAO, Jie YU, Ping ZHENG, Hong CHEN Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China College of Food Science, Sichuan Agricultural University, Yaan, Sichuan, P....
متن کاملProkaryotic Expression of Influenza A virus Nucleoprotein Fused to Mycobacterial Heat Shock Protein70
Background and Aims: The novel approaches in influenza vaccination have targeted more conserved viral proteins such as nucleoprotein (NP) to provide cross protection against all serotypes of influenza A viruses. Influenza specific cytotoxic T lymphocytes (CTL) are able to lyse influenza-infected cells by recognition of NP, the major target molecule in virus for CTL responses. On the other hand,...
متن کاملDistributed Computing with Prokaryotic Immune Systems
We propose the in vivo/vitro use of prokaryotic adaptive immune systems for distributed learning. In the coming years synthetic biologists will learn to control, program, and modify such systems. We design an enhancement to CRISPR/Cas immune systems and demonstrate the learning potential of the modified system by showing that it can approximate solutions to a computationally hard problem. To ou...
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ژورنال
عنوان ژورنال: Postępy Higieny i Medycyny Doświadczalnej
سال: 2013
ISSN: 1732-2693
DOI: 10.5604/17322693.1038351